Jayshree Samanta, MBBS, PhD

jayshree.samanta@wisc.edu

Department of Comparative Biosciences
Office: 3354C
Website

Jayshree Samanta, MBBS, PhD

Titles and Education

  1. M.B.B.S., Grant Medical College, University of Mumbai, India.
  2. Ph.D. (Neuroscience), Northwestern University, Chicago, USA
  3. Post-doctoral Fellow (Neuroscience), New York University, New York, USA

Research

Visit the Samanta Lab

Our lab focuses on how neural stem cells generate myelin in the brain during development as well as during recovery from a demyelinating insult i.e. remyelination. Our primary goal is to understand the disease process and identify factors that can help neural stem cells repair the brains with myelin abnormalities as observed in diseases like Multiple Sclerosis.

The myelin sheath is a specialized membrane synthesized by oligodendrocytes, which wraps around the axons of neurons in the vertebrate brain. In demyelinating diseases, disruption of myelin results in severe neurological defects due to conduction block ultimately leading to the loss of axons. The goal in inherited myelin diseases is to myelinate axons that have not been ensheathed; while in demyelinating diseases, the goal is to ensheath the axons that have lost their myelin. Our objective is to understand the molecular underpinnings of signaling pathways with respect to their role in proliferation, migration and differentiation of neural stem cells during developmental myelination and remyelination.

We use several techniques to study myelin generation from neural stem cells:

1. Mouse models of diseases: To study remyelination, we induce demyelination in the brain with Lysolecithin injection, Cuprizone diet or use genetically modified mice responsive to Diphtheria toxin. 

2. In vivo mouse genetics: We label specific populations of neural stem cells in the brain using genetically inducible fate-mapping techniques like Cre-Lox recombination. We also use genetically modified mice to activate or inhibit specific signaling pathways in neural stem cells by overexpressing or ablating different components of the pathway.

3. In vitro neural stem cell culture: We harvest neural stem cells from the ganglionic eminences in embryonic brains and subventricular zone in adult mouse brains and grow them as suspension cultures in a dish. In addition, we manipulate the signaling pathways pharmacologically or genetically to study proliferation and differentiation of neural stem cells in vitro.

Responsibilities

Assistant Professor

                      Veterinary Neuroanatomy and Neurophysiology

Graduate Training

Cellular and Molecular Biology Graduate Program

Cellular and Molecular Pathology Graduate Program

Comparative Biomedical Sciences Graduate Program

Molecular and Cellular Pharmacology Graduate Program

Recent Publications

  1. DZ Radecki, J Samanta. Endogenous Neural Stem Cell Mediated Oligodendrogenesis in the Adult Mammalian Brain. (2022) Cells, 11(13):2101, https://doi.org/10.3390/cells11132101
  2. DZ Radecki, J SamantaIsolation and culture of neural stem cells from adult mouse subventricular zone for genetic and pharmacological treatments with proliferation analysis. (2022) Star Protocols, PMID: 35146452
  3. DZ Radecki, AR Wang, AS Johnson, CA Overman, MM Thatcher, G Iyer, J SamantaGpnmb inhibits oligodendrocyte differentiation of neural stem cells by amplifying TGFb1 signaling (2021). bioRxiv 2021.08.13.456269
  4. B Zotter, O Dagan, J Brady, H Baloui, J Samanta, JL Salzer. Gli1 regulates the postnatal acquisition of peripheral nerve architecture (2021). Journal of NeurosciencePMID: 34772739
  5.  J Samanta, HM Silva, JJ Lafaille, JL Salzer. Transcriptomic analysis of loss of Gli1 in neural stem cells responding to demyelination in the mouse brain (2021). Scientific Data PMID: 34711861
  6. DE Marzan, V Verdon, BL West, S Liddelow, J Samanta, JL Salzer. Activated microglia drive demyelination via CSF1R signaling, (2021)  Glia, PMID: 33620118.
  7. DZ Radecki, HM Messling, JR Haggerty-Skeans, SK Bhamidipati, ED Clawson, CA Overman, MM Thatcher, JL Salzer, J Samanta. Relative Levels of Gli1 and Gli2 Determine the Response of Ventral Neural Stem Cells to Demyelination. (2020) Stem Cell Reports, PMID: 33125874.
  8.  JL Salzer, J Samanta, GJ Fishell. Method for enhancing remyelination using GLI1 inhibitors (2016) US Patent 9,248,128
  9. J Samanta, EM Grund, HM Silva, JJ Lafaille, G Fishell, JL Salzer. Inhibition of Gli1 mobilizes endogenous neural stem cells for remyelination. (2015) Nature, PMID: 26416758.
  10. J Samanta, JL Salzer. Myelination: actin disassembly leads the way. (2015) Developmental Cell , PMID: 26218317.
  11. J Samanta, T Alden, K Gobeske, L Kan, JA Kessler. Noggin protects against ischemic brain injury in rodents. (2010) Stroke , PMID: 20019326
  12. J Samanta, JA Lyons. Keystone Symposium on Multiple Sclerosis. (2009) Future Medicine 4 (3), 279-281
  13. J Samanta, MA Bonaguidi, JA Kessler. The TGF-beta family in neural and neuronal differentiation and development (2008) COLD SPRING HARBOR MONOGRAPH SERIES 50, 819
  14.  J Samanta, GM Burke, T McGuire, AJ Pisarek, A Mukhopadhyay, Y Mishina, JA Kessler. BMPR1a signaling determines numbers of oligodendrocytes and calbindin-expressing interneurons in the cortex (2007) Journal of Neuroscience, PMID: 17626200
  15. MA Bonaguidi, T McGuire, M Hu, L Kan, J Samanta, JA Kessler. LIF and BMP signaling generate separate and discrete types of GFAP-expressing cells (2005) Development , PMID: 16314487
  16. J Samanta, JA Kessler. Interactions between ID and OLIG proteins mediate the inhibitory effects of BMP4 on oligodendroglial differentiation (2004)  Development , PMID: 15280210
  17. U Guha, WA Gomes, J Samanta, M Gupta, FL Rice, JA Kessler. Target-derived BMP signaling limits sensory neuron number and the extent of peripheral innervation in vivo (2004) Development , PMID: 14973275.