Titles and Education

1. Marquette University, B.S., Biology, 1978
2. University of Illinois at Urbana-Champaign, Ph.D., Physiology and Biophysics, 1984
3. Postdoctoral training: Dept. of Obstetrics and Gynecology, Univ. of Pennsylvania, 1984-87
4. Professor (2007-present) and Chair (2015-2021), Comparative Biosciences, UW School of Veterinary Medicine
5. Joint Faculty Appointment, Obstetrics and Gynecology, UW School of Medicine and Public Health
6. Co-chair, Regenerative and Reproductive Medicine Group, Wisconsin National Primate Research Center
7. Co-head, Precision Medicine and Genomic Resources Unit, Wisconsin National Primate Research Center

Research

VISIT THE GOLOS LAB WEBSITE

My laboratory examines the questions of placental biology relevant to human health and disease.  We use nonhuman primate models in our studies.  Under this broad focus, research areas include

• maternal-fetal immune dialogue in establishment of a healthy pregnancy, and protection of the fetus from bacterial and viral infection
• IVF-derived primate embryos for genomic editing to generate animal models of human genetic diseases, and to model implantation and placental development.

Our studies of embryo/maternal interactions will lead to improved understanding of the establishment and maintenance of pregnancy, reasons for early pregnancy loss, and insights into the role(s) of the placenta in promoting fetal well-being.  Appropriate placental development underpins appropriate fetal growth as well as maternal health and well-being.  A detrimental intrauterine environment can profoundly affect postnatal physiology throughout the lifespan.

Responsibilities

Veterinary Education

• Veterinary Histology 934:501

Graduate Training

• Endocrinology–Reproductive Physiology Training Program
• Stem Cell and Regenerative Medicine Training Program
• Comparative Biomedical Sciences Training Program
• Cellular and Molecular Pathology Training Program

Recent Publications

1. Burleigh, D.W., C.M. Kendziorski, Y.J. Choi, K.M. Grindle, R.L. Grendell, R.R. Magness and T.G. Golos. 2007. Microarray analysis of BeWo and JEG3 trophoblast cell lines: identification of differentially expressed transcripts. Placenta 28:383-389.

2. Bondarenko G.I., Burleigh D.W., Durning M., Breburda E.E., Grendell R.L., and T.G. Golos. 2007. Passive Immunization against the MHC Class I Molecule Mamu-AG Disrupts Rhesus Placental Development and Endometrial Responses. J. Immunol. 179:8042-8050.

3. Dambaeva, S.V., Bondarenko, G.I., Grendell R.L., Kravitz, R.H., Durning, M., and T.G. Golos. 2008. Nonclassical MHC-E (Mamu-E) expression in the rhesus monkey placenta. Placenta 29:58-70. PMCID: PMC2754872

4. Douglas, G.C., C.A. VandeVoort, P. Kumar, T.C. Chang, and T.G. Golos, 2009. Trophoblast stem cells: Models for Investigating Trophectoderm Differentiation and Placental Development. Endocrine Reviews. 30:228-40. PMCID: PMC2726840

5. Thomas C.P., J.I. Andrews, N.S. Raikwar, E.A. Kelley, F. Herse, R. Deschend, T.G. Golos, and K.Z. Liu. 2009. A recently evolved novel trophoblast-enriched secreted form of fms-like tyrosine kinase-1 variant is upregulated in hypoxia and in preelcampsia. J. Clin. Endo. Metab. 94:2524-2530. PMCID: PMC2708964

6. Rozner, A.E., Dambaeva, S.V., Drenzek, J.G., Durning, M., and Golos, T.G.  2009 Generation of macrophages from peripheral blood monocytes in the rhesus monkey. J. Immunol. Methods 351:36-40. PMCID: PMC3564589

7. Golos, T.G., Bondarenko G.I., Dambaeva, S.V., Breburda, E.E., and M. Durning. 2010. On the role of placental major histocompatibility complex and decidual leukocytes in implantation and pregnancy success using non-human primate models. Int. J. Dev. Biol. 54:431-443. Review. PMCID: PMC3069127

8. Giakoumopoulos, M., Siegfried, L.M. Dambaeva, S.V., Garthwaite, M.A., and T.G. Golos. 2010 Placental-derived mesenchyme influences chorionic gonadotropin and progesterone  secretion of human embryonic stem cell-derived trophoblasts. Reproductive Sciences. 17(9):798-808. NIHMSID: NIHMS282437. PMCID: PMC3065864

9. Rozner, A.E., Dambaeva, S.V., Drenzek, J.G., Durning, M., and Golos, T.G. 2011. Modulation of Cytokine and Chemokine Secretion in Rhesus Monkey Trophoblast Co-Culture with Decidual but not Peripheral Blood Monocyte-Derived Macrophages. Am. J. Reprod. Immunol. 66:115-127. PMCID: PMC3132823.

10. S.V. Dambaeva, M. Durning, A.E. Rozner, and T.G. Golos. 2012. Immunophenotype and Cytokine Profiles of Rhesus Monkey CD56bright and CD56dim Decidual Natural Killer (NK) Cells. Biol. Reprod. 86: 1-10. PMCID: PMC3313663

11. Bondarenko, GI, Durning M, Golos TG. 2012. Immunomorphological changes of rhesus monkey endometrium during the menstrual cycle and early pregnancy. Am. J. Reprod. Immunol. 68:309-21.  PMCID:  PMC3440518

12. Giakoumopoulos M, Golos TG. 2013. Embryonic stem cell-derived trophoblast differentiation: a comparative review of the biology, function, and signaling mechanisms. J. Endocrinol. 216: R33-R45.

13. Poulsen KP, Faith NG, Golos TG, Giakoumopoulos M, Czuprynski CJ. 2014 Listeria monocytogenes infection reduces the functionality of human choriocarcinoma JEG-3 cells. J. Neonatal Biol. 3:125. doi: 10.4172/2167-0897.1000125.

14. Iruretagoyena JI, Davis W, Bird C, Olsen J, Radue R, Broman AT, Kendziorski C, Bondurant SS, Golos T, Bird I, Shah D. 2014 Differential changes in gene expression in human brain during late first trimester and early second trimester of pregnancy. Prenat Diagn. 34: 431-437.

15. Prabhudas M, et al. 2015. Immune mechanisms at the maternal-fetal interface: perspectives and challenges. Nat. Immunol. 16: 328-34.

16. Dudley, DM et al. 2016. A rhesus macaque model of Asian lineage Zika virus infection. Nat Comm. 7:12204 / DOI: 10.1038/ncomms12204.
17. Rozner AM, Durning M, Kropp J, Wiepz GJ, Golos TG. Decidual Macrophages Influence the Differentiation of Rhesus Monkey Embryonic Trophoblasts. 2016. Am. J. Reprod. Immunol. 76:364-375.

18. Chapters in Books

19. Golos, T.G. 2011. Stem Cells From the Placenta. In: The Placenta: from Development to Disease. Wiley-Blackwell, H. Kay, D. Michael Nelson and Y. Wang (eds), West Oxford, UK.

20. Hélène Marquis, Douglas A. Drevets, Michael S. Bronze, Sophia Kathariou, Thaddeus G. Golos, J. Igor Iruretagoyena. 2015. Pathogenesis of Listeria monocytogenes in human. In: Emerging and Re-emerging Human Infections. John Wiley & Sons/Wiley Blackwell Press, pp 747-770.

21. Wolfe KB, Wiepz GJ, Schotzko ML, Bondarenko GI, Durning M, Faith NG, Simmons HA, Mejia A, Suresh M, Czuprynski CJ, Kathariou S, Golos TG. 2017. Acute fetal demise in early nonhuman primate pregnancy infection with Listeria monocytogenes. mBio 8: e01938-16.

22. Nguyen SM, Antony KM, Dudley DM, Kohn S, Simmons HA, Wolfe B, Salamat MS, Teixeira LBC, Wiepz GJ, Thoong TH, Aliota MT, Weiler AM, Barry GL, Weisgrau KL, Vosler LJ, Mohns MS, Breitbach ME, Stewart LM, Rasheed MN, Newman CM, Graham ME, Wieben OE, Turski PA, Johnson KM, Post J, Hayes JM, Schultz-Darken N, Schotzko ML, Eudailey JA, Permar SR, Rakasz EG, Mohr EL, Capuano III S, Tarantal AF, Osorio JE, O’Connor SL, Friedrich TC, O’Connor DH, Golos TG.  2017.  Highly EfficientMaternal-Fetal Zika Virus Transmission in Pregnant Rhesus Macaques.  PLoS Pathog 13(5): e1006378. https://doi.org/10.1371/journal.ppat.1006378

23. Vermilyea SC, Guthrie S, Meyer M, Smuga-Otto K, Braun K, Howden S, Thomson JA, Zhang SC, Emborg ME, Golos TG.  2017.  Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Adult Common Marmoset Fibroblasts.  Stem Cells and Development 26(17):1225-1235. doi: 10.1089/scd.2017.0069.

24. Kropp J, Block LN, Golos TG. 2018. Defining the rhesus macaque placental miRNAome: conservation of expression of placental miRNA clusters between the macaque and human. Placenta doi: 10.1016/j.placenta.2018.04.003.

25. Mohr EL, Block LN, Newman CM, Stewart LM, Koenig M, Semler M, Breitbach ME, Teixeira LBC, Zeng X, Weiler AM, Barry GL, Thoong TH, Wiepz GJ, Dudley DM, Simmons HA, Mejia A, Morgan TK, Salamat MS, Kohn S, Antony KM, Aliota MT, Mohns MS, Hayes JM, Schultz-Darken N, Schotzko ML, Peterson E, Capuano S 3rd, Osorio JE, O’Connor SL, Friedrich TC, O’Connor DH, Golos TG.  2018. Ocular and uteroplacental pathology in a macaque pregnancy with congenital Zika virus infection.  PLoS One. Jan 30;13(1):e0190617. Doi: 10.1371/journal.pone.0190617.eCollection 2018.

26. Dudley DM, Van Rompay KK, Coffey LL, Ardeshir A, Keesler RI, Grigsby PL, Steinbach RJ, Hirsch AJ, MacAllister RP, Hodge T, Streblow DN, Tardif S, Patterson JL, Tamhankar M, Seferovic M, Aagaard KM, Sanchez-San Martin C, Chiu CY, Panganiban AT, Maness NJ, Gilbert MH, Bohm RP, Wang X, Adams Waldorf KM, Gale Jr M, Rajapopal L, Hotchkiss CE, Mohr EL, Capuano III SV, Friedrich TC, Golos TG, O’Connor DH.  2018. Miscarriage and Stillbirth Following Maternal Zika Virus Infection in Nonhuman Primates.  Nature Medicine: https://doi.org/10.1038/s41591-018-0088-5.

27. Macdonald JA, Corrado PM, Nguyen SM, Johnson KM, Francois CJ, Magness RR, Shah DM, Golos TG, Wieben OE. 2019 Uteroplacental and Fetal 4D Flow MRI in the Pregnant Rhesus Macaque. J. Mag Res Imag. 2019 Feb;49(2):534-545.  doi: 10.1002/jmri/26206.

28. Banerjee P, Ries M, Janaka S, Grandea III AG, Wiseman R, O’Connor DH, Golos TG, Evans DT.  Diversification of Bw4 Specificity and Recognition of a Non-Classical MHC Class I Molecule Implicated in Maternal-Fetal Tolerance by Killer-Cell Immunoglobulin-Like Receptors of the Rhesus Macaque. 2018 J Immunol. 201: 2776-2786.

29. Nguyen SM, Wiepz GJ, Schotzko ML, Simmons HA, Mejia A, Ludwig KD, Zhu A, Brunner KA, Hernando D, Reeder SB, Wieben O, Shah DM, Johnson K, Golos TG. 2020. Impact of Ferumoxytol Magnetic Resonance Imaging on the Rhesus Macaque Maternal-Fetal Interface. Biology of Reproduction. 102:434-444.
30. Block LN, Aliota MT, Friedrich TC, Schotzko ML, Mean KD, Wiepz GJ, Golos TG, Kropp Schmidt J. 2020 Embryotoxic impact of Zika virus in a rhesus macaque in vitro implantation model. Biology of Reproduction. Apr 15;102(4):806-816. doi: 10.1093/biolre/ioz236.
31. Vermilyea SC, Babinski A, Tran N, To S, Guthrie S, Kluss JH, Kropp Schmidt J, Wiepz GJ, Meyer MG, Murphy ME, Cookson MR, Emborg ME, Golos TG.  In Vitro CRISPR/Cas9-2020 Directed Gene Editing to Model LRRK2 G2019S Parkinson’s Disease in Common Marmosets.  Feb 26;10(1):3447. doi: 10.1038/s41598-020-60273-2.
32. Schmidt JK, Strelchenko N, Park MA, Kim YH, Mean KD, Schotzko ML, Kang HJ, Golos TG, Slukvin II. 2020. CRISPR-Cas9 editing of CCR5 in Mauritian cynomolgus macaque embryos. Sci Rep 10: 18457.
33. Schmidt JK, Mean KD, Puntney RC, Alexander ES, Sullivan R, Simmons HA, Zeng X, Weiler AM, Friedrich TC, Golos TG. Zika virus in rhesus macaque semen and reproductive tract tissues with acute infection. 2020 Biology of Reproduction: 103(5):1030-1042.
34. Block LN, Bowman BD, Keding LT, Schmidt JK, Stanic AK, Golos TG. The promise of placental extracellular vesicles: models and challenges for diagnosing placental dysfunction in utero. 2020 Biology of Reproduction: 2020 Aug 28:ioaa152. doi: 10.1093/biolre/ioaa152
35. Schmidt JK, Keding LT, Block LN, Wiepz GJ, Koenig MR, Meyer MG, Dusek BM, Kroner KM, Bertogliat MJ, , Kallio AR, Mean KD, Golos TG. 2020 Placenta-derived macaque trophoblast stem cells: differentiation to syncytiotrophoblasts and extravillous trophoblasts reveals phenotypic reprogramming. Scientific Reports: 10(1):19159, 2020.