Titles and Education

1. 1. B.S. in Biology, St. Norbert College, 2010

   2. Ph.D. in Comparative Biosciences, University of Wisconsin-Madison, 2014

   3. Postdoctoral research in neurotoxicology, University of California-Davis, 2015-2019

Research

Our lab is focused on understanding the impact of the environment on the establishment and regulation of urinary function. In particular, we are focused on developmental exposure to environmental contaminants. One environmental contaminant of interest includes polychlorinated biphenyls (PCBs). PCBs are ubiquitous environmental contaminants, and while they are known to have deleterious effects on the developing brain and central nervous system, their effects on the peripheral nervous system and peripheral target tissues - such as the bladder - are not understood. Therefore, we are using a combination of in vitro and in vivo approaches in mice to identify the effects of PCBs on urinary function focusing not only on bladder innervation and physiology but also on input from the central nervous system and dorsal root ganglia which are critical in controlling urinary function. Our ultimate goal is to be able to identify targets for therapeutic intervention and/or identify critical windows of exposure to help mitigate risk.

Responsibilities

Assistant Professor

-Veterinary Toxicology (Course Director)

Graduate Training

Comparative Biomedical Sciences
Molecular and Environmental Toxicology
Endocrinology and Reproductive Physiology

Recent Publications

1. 1. Lavery T, Spiegelhoff A, Wang K, Kennedy CL, Ridlon M Keil Stietz KP. (2023). Polychlorinated biphenyl (PCB) exposure in adult female mice can influence bladder contractility. Am J Clin Exp Urol. 11(5):367-384. PMCID: PMC10628623.
2. 1. Spiegelhoff A, Wang K, Ridlon M, Lavery T, Kennedy CL, George S, Keil Stietz KP. (2023). Polychlorinated biphenyls (PCBs) impact prostatic collagen density and bladder volume in young adult mice exposed during in utero and lactational development. Toxics. 11(7): 609. PMCID: PMC10384510.
3. 1. Zhang CY, Li X, Keil Stietz K, Sethi S, Yang W, Marek R, Ding X, Lein P, Hornbuckle K, Lehmler, H-Joachim. (2023). Rebuttal to Correspondence on Machine Learning-Assisted Identification and Quantification of Hydroxylated Metabolites of Polychlorinated Biphenyls in Animal Sample. Environ Sci Technol. Apr 18;57(15):6251-6252. PMID: 37017328.
4. 1. Zhang CY, Li X, Keil Stietz K, Sethi S, Yang W, Marek R, Ding X, Lein P, Hornbuckle K, Lehmler, H-Joachim. (2022). Machine learning-assisted identification and quantification of hydroxylated metabolites of polychlorinated biphenyls in animal samples. Environ Sci Technol. Sep 20;56(18):13169-13178. PMCID: PMC9573770.
5. 1. Kennedy CL, Spiegelhoff A, Lavery T, Wang K, Manuel RJ, Wang Z, Wildermuth H, Keil Stietz K. (2022). Developmental polychlorinated biphenyl (PCB) exposure alters voiding physiology in young adult male and female mice. Am J Clin Exp Urol. 10(2):82-97. PMCID: PMC9077147
6. Kennedy, C.L., Spiegelhoff, A., Wang, K., Lavery, T., Nunez, A., Manuel, R., Hillers-Ziemer, L., Arendt, L.M., Stietz, K.P.K. (2021). The bladder is a novel target of developmental polychlorinated biphenyl exposure linked to increased inflammatory cells in the bladder of young mice. Toxics, 9(9):214. https://doi.org/10.3390/toxics9090214. PMCID- PMC8473463.
7. Keil Stietz KP*, Kennedy CL, Sethi S, Valenzuela A, Nunez A, Wang K, Wang, Z, Wang P, Speigelhoff A, Puschner B, Bjorling DE, Lein PJ. (2021). In utero and lactational PCB exposure drives anatomic changes in the juvenile mouse bladder. Current Research in Toxicology. 2:1-18. PMCID-PMC8317607. *Corresponding authorship.
8. Abler LL, O’Driscoll CA, Colopy SA, Keil Stietz KP, Wang P, Wang Z, Hartmann F, Crader-Smith SM, Oellete JN, Mehta V, Oakes S, Grimes MD, Mitchell GS, Baan M, Gallagher SJ, Davis D, Kimple ME, Bjorling DE, Watters JJ, Vezina CM. (2021). The influence of intermittent hypoxia, obesity and diabetes on male genitourinary anatomy and voiding physiology. American Journal of Physiology Renal. In Press. https://doi.org/10.1152/ajprenal.00112.2021.
9. Turco AE, Oakes SR, Keil Stietz KP, Dunham CL, Joseph DB, Chathurvedula TS, Girardi NM, Schneider AJ, Gawdzik J, Sheftel CM, Wang P, Wang Z, Bjorling DE, Ricke WA, Tang W, Hernandez LL, Keast JR, Bonev AD, Grimes MD, Strand DW, Tykocki NR, Tanguay RL, Peterson RE, Vezina CM. (2021). A neuroanatomical mechanism linking perinatal TCDD exposure to lower urinary tract dysfunction in adulthood. Disease Models and Mechanisms, 14(7). https://doi.org/10.1242/dmm.049068. PMCID- PMC8326766.
10. Keil Stietz KP., Sethi S., Klocke CR., de Ruyter T., Wilson MD., Pessah I., Lein PJ. (2021). Sex and Genotype Modulate the Dendritic Effects of Developmental Exposure to a Human-Relevant Polychlorinated Biphenyls Mixture in the Juvenile Mouse. Frontiers in Neuroscience, 15:1610. 10.3389/fnins.2021.766802.
11. Sethi S., Keil Stietz KP*., Valenzuela AE., Klocke CR., Silverman JL., Puschner B., Pessah IN., Lein PJ. (2021) Developmental Exposure to a Human-Relevant PCB Mixture Causes Behavioral Phenotypes that Vary by Sex and Genotype in Juvenile Mice Expressing Human Mutations that Modulate Neuronal Calcium. Frontiers in Neuroscience, 15:1689. 10.3389/fnins.2021.766826. *Co-first authorship.
12. Panesar HK, Kennedy CL, Keil Stietz KP*, Lein PJ*. (2020). Polychlorinated Biphenyls (PCBs): Risk Factors for Autism Spectrum Disorder?. Toxics. 17;8(3):E70. PMCID-in process. *Co-corresponding authorship.
13. Matelski L, Keil Stietz KP, Sethi S, Taylor SL, Van de Water J, Lein PJ. (2020). The Influence of Sex, Genotype, and Dose on Serum and Hippocampal Cytokine Levels in Juvenile Mice Developmentally Exposed to a Human-Relevant Mixture of Polychlorinated Biphenyls. Current Research in Toxicology. 1:85-103. doi: https://doi.org/10.1016/j.crtox.2020.09.001.

14. Wang Z, Guzman EC, Nimunkar A, Keil KP, Vezina CM, Ricke WA, Macoska J, Bjorling DE (2019). Void Sorcerer: an Open Source, Open Access Framework for Mouse Uroflowmetry. Am J Clin Exp Urol. 7(3):170-177. PMCID - PMC6627548

15. Rude K, Pusceddu MM, Keogh CE, Sladek JA, Rabasa G, Miller EM, Sethi S, Keil KP, Pessah IN, Lein PJ, Gareau MG. (2019). Developmental exposure to polychlorinated biphenyls (PCBs) in the maternal diet causes host-microbe defects in weanling offspring mice. Environmental Pollution. 253:708-721.

16. Keil KP, Sethi S, Lein PJ. (2018). Sex differences in dendrite morphology in response to 2,2′,3,5′,6-pentachlorobiphenyl (PCB 95) in cultured primary mouse neurons. Tox Sci. doi: 10.1093/toxsci/kfy277

17. Keil KP, Sethi S, Wilson MD, Silverman JS, Pessah IN, Lein PJ. (2018). Genetic mutations in Ca2+ signaling alter dendrite morphology and social approach in juvenile mice. Genes Brain Behav. e12526. PMCID – PMC6540090

18. Sethi S, Keil KP, Lein PJ. (2018). 3,3ʹ-Dichlorobiphenyl (PCB 11) Promotes Dendritic Arborization in Primary Rat Cortical Neurons via CREB-Dependent Mechanisms. Arch Toxicol. 92(11):3337-3345. PMCID - PMC6196112.

19. Keil KP, Sethi S, Wilson MD, Chen H, Lein PJ (2017). Dendritic morphology varies between sexes in juvenile mice and in vitro models of neurodevelopment. Sci Rep. 7(1): 8486. PMCID – PMC5559594.

20. Wilson MD, Sethi S, Lein PJ, Keil KP. (2017). Valid statistical approaches for analyzing sholl data: mixed effects versus simple linear models. J. Neurosci Methods. 279:33-43. PMCID - PMC5346342

21. Ricke WA, Lee C, Clapper T, Schneider AJ, Moore RW, Keil KP, Abler LL, Wynder JL, Alvarado AL, Beaubrun I, Vo J, Bauman TM, Ricke EA, Peterson RE, Vezina CM (2016). In utero and lactational TCDD exposure increases susceptibility to lower urinary tract dysfunction in adulthood. Tox Sci. 50(2):429-40. PMCID - PMC26865671.

22. Keil KP, Abler LL, Altmann HM, Wang Z, Wang P, Ricke WA, Bjorling DE, Vezina CM. (2015). Impact of a folic acid enriched diet on lower urinary tract function in mice treated with testosterone and estradiol. Am J Physiol Renal Physiol. 308(12): 1431-43. PMCID - PMC4469891.