Department of Pathobiological Sciences
Office: 119 AHABS
Universidad de Antioquia (Colombia)D.V.M.1985 Veterinary Medicine
University of Wisconsin M.S. 1988 Veterinary Science
University of Wisconsin Ph.D. 1996 Animal Health and Biomedical Science
Positions and Employment:
1985-1988 Research Assistant, University of Wisconsin, Madison, WI
1987-1992 Field Scientist, USAMRIID
1988-1996 Research Assistant, University of Wisconsin, Madison, WI
1996 Postdoctoral Fellow, University of Wisconsin, Madison, WI
1996-1998 Senior Scientist, R&D Molecular Vaccines, Heska Corporation, Fort Collins, CO
1998 Affiliate Faculty, Colorado State University, Fort Collins, CO
1998-2000 Senior Research Microbiologist, Merial Limited, Athens, GA
2000-2004. Scientist, PowderJect Vaccines Inc., Madison, WI
2003- Chief Scientific Officer, Inviragen, Madison, WI
2004- Assistant Professor, Dept. of Pathobiological Sciences,
School of Veterinary Medicine, Univ. of Wisconsin-Madison
Matricula de Honor (academic scholarship), Universidad de Antioquia.
Orlando Vasquez Arango Award. Universidad de Antioquia.
P90038US. Attenuation of Cardioviruses in non-murine mammals. 1993
6106841. Novel delivery method for recombinant raccoon poxvirus. 2000
To contribute to the knowledge and understanding of the pathogenesis of important human and veterinary diseases and to develop novel methods for vaccination and prevention. Strongest interest on viruses and zoonotic pathogens. We are establishing at UW-Madison a state-of-the-art research group that uses molecular approaches to unravel host-pathogen interactions for emerging diseases (e.g dengue, West Nile) and biodefense pathogens (e.g Monkeypox, plague). Our group is actively researching and developing novel human and animal vaccines for diseases including highly pathogenic avian influenza, dengue, chikungunya and plague. We are committed to mentoring and guiding young bright students and to improving veterinary and human public health in developing countries, especially in Latin America.
Current lab members:
Willy Berlier (Post Doctoral Fellow): Pathogenesis and vaccines for Yersinia pestis (working at the USGS-National Wildlife Health Center).
Joe Brewoo (Scientist): Immunology of vaccine: Research into immunogenecity of chimeric dengue vaccines and pox based vaccines for plague, influenza and Chikungunya.
Harry Partidos (Senior Scientist): Expert immunologist in vaccine research esp. Chikungunya, dengue and influenza.
Brock Bakke (Graduate Student): Engineering and molecular biology of pox based vaccines for Highly Pathogenic Avian Influenza, Canine influenza and Plague. Running BL3 facilities and conducting BL3 challenge studies for Chikungunya and Influenza.
Liz Falendysz (DVM/MS student): IHC and pathogenesis of Pox Virus. Conducting challenge studies for RCN based plague vaccines.
Andrea Carlson (DVM/MS student): Feild studies for west-nile like viruses.
Karl Aponte (DVM): Field studies for influenza, and evaluation of RCN based vaccines in fowl.
Monica Restrepo Garcia (Research Intern): Immunology of Chikungunya vaccine induced cytokines.
Previous lab members:
Dr. Keith P. Iams. Dr. Sandra Dawe, Dr. Jennifer Dolan-Livengood, Christina Hutson Yenny Goez, Joseph Campbell, Mauricio Barajas. Angela Londoño Navas. Shi-Hsia Hwa. Diana Piedrahita. Adam Ericsen.
1. Duke GM, Osorio JE, Palmenberg AC. Attenuation of Mengo virus through genetic engineering of the 5' noncoding poly(C) tract. Nature 1990;343(6257):474-476.
2. Linthicum KJ, Logan TM, Bailey CL, Gordon SW, Peters CJ, Monath TP, Osorio JE, Francy DB, McLean RG, Leduc JW, et al. Venezuelan equine encephalomyelitis virus infection in and transmission by the tick Amblyomma cajennense (Arachnida: Ixodidae). J Med Entomol1991; 28(3):405-409.
3. Osorio JE, Godsey MS, Defoliart GR, Yuill TM. La Crosse viremias in white-tailed deer and chipmunks exposed by injection or mosquito bite. Am J Trop Med Hyg 1996;54(4):338-342.
4. Osorio JE, Yuill TM. Venezuelan equine encephalitis. Chapter, in: Handbook of Zoonoses. Section B: Viral. Second edition. CRC Press. 1994
5. Osorio JE, Yuill TM. La Crosse viremias in Mongolian gerbils (Meriones unguiculatis). Am J Trop Med Hyg 1996;55(5):567-569.
6. Osorio JE, Schoepp RJ, Yuill TM. Effects of La Crosse virus infection on pregnant domestic rabbits and mongolian gerbils. Am J Trop Med Hyg 1996;55(4):384-390.
7. Osorio JE, Martin LR, Palmenberg AC. The immunogenic and pathogenic potential of short poly(C) tract Mengo viruses. Virology 1996;223(2):344-350.
8. Osorio JE, Godsey MS, Defoliart GR, Yuill TM. La Crosse viremias in white-tailed deer and chipmunks exposed by injection or mosquito bite. Am J Trop Med Hyg 1996;54(4):338-342.
9. Martin LR, Duke GM, Osorio JE, Hall DJ, Palmenberg AC. Mutational analysis of the mengovirus poly(C) tract and surrounding heteropolymeric sequences. J Virol 1996;70(3):2027-2031.
10. Osorio JE, Hubbard GB, Soike KF, Girard M, van der Werf S, Moulin JC, Palmenberg AC. Protection of non-murine mammals against encephalomyocarditis virus using a genetically engineered Mengo virus. Vaccine 1996;14(2):155-161.
11. Osorio JE, Tomlinson CM, Frank RS, Haanes B, Rushlow K, Haynes J R, Stinchcomb DT. Immunization of dogs and cats with a nucleic acid-based vaccine against rabies virus. Vaccine 1999;17: 1109-116.
12. Osorio JE, Grossberg SE, Palmenberg AC. Characterization of genetically engineered Mengoviruses in mice. Viral Immunol 2000;13:27-35.
13. Chen D, Weis KF, Chu Q, Erickson C, Endres R, Lively CR, Osorio J, Payne LG. Epidermal powder immunization induces both cytotoxic T-lymphocyte and antibody responses to protein antigens of influenza and hepatitis B viruses. J Virol. 2001;75(23):11630-40.
14. Chen D, Zuleger C, Chu Q, Maa YF, Osorio JE, Payne LG. Epidermal powder immunization with a recombinant HIV gp120 targets Langerhans cells and induces enhanced immune responses. AIDS Res Hum Retroviruses. 2002;18(10):715-22.
15. Osorio JE, Powell TD, Frank RS, Moss K, Haanes EJ, Smith SR, Rocke TE, Stinchcomb DT. Recombinant raccoon pox vaccine protects mice against lethal plague. Vaccine 2003; 7(21):1232-38.
16. Osorio JE, Zuleger CL, Burger M, Chu Q, Payne LG, Chen D. Immune responses to hepatitis B surface antigen following epidermal powder immunization. Immunol Cell Biol. 2003;81(1):52-8.
17. Maa Y-F, Shu C, Ameri M, Zuleger C, Che J, Osorio JE, Payne LG, and Chen D. Optimization of an alum-adsorbed vaccine powder formulation for epidermal powder immunization. Pharmaceutical Research. 2003 (20):969-77.
18. Dean HJ, Fuller D, Osorio JE. Powder and particle-mediated approaches for delivery of DNA and protein vaccines into the epidermis. Comp Immunol Microbiol Infect Dis. 2003;26(5-6):373-88.
19. Osorio JE, Frank RS, Moss K, Taraska, T, Powell, and Stinchcomb DT. Raccoon pox as a mucosal vaccine vector for domestic cats. Journal of Drug Targeting. 2003 (8-10): 463-470.
20. Rocke TE, Dein FJ, Fuchsberger M, Fox BC, Stinchcomb DT, Osorio JE. Limited infection upon human exposure to a recombinant raccoon pox vaccine vector. Vaccine 2004 (22): 2757-2760.
21. Mencher J, Rocke TE, Smith SR, Osorio JE, Stinchcomb DT. Protection of black-tailed prairie dogs (Cynomys ludovicianus) against plague after voluntary consumption of baits containing recombinant raccoon poxvirus vaccine. Infect Immun. 2004 Sep;72(9):5502-5.
22. Osorio JE, Che J, Guzman JL, Payne LG, Chen D, and Carlin N. Increased efficacy of influenza vaccines by cholera toxin B subunit (rCTB) in mice. (Manuscript in preparation).
23. Maa Y-F, Shu C, Zuleger C, Che J, Osorio JE, Payne LG, and Chen D. Hepatits-B surface Antigen (HbsAg) powder formulation: Process and Stability assessment (Submitted for publication)