Education

• Professor of Microbiology
• BS 1970, University of Minnesota
• DVM 1972, University of Minnesota
• PhD 1976, University of Georgia
• Diplomate, American College of Veterinary Microbiologists

Research

Paratuberculosis (Johne's disease) and the biology of its causative agent, Mycobacterium paratuberculosis. Johne's disease is a prevalent, costly infectious disease affecting dairy cattle and other ruminants. Improving methods of detecting the disease is the primary focus of the laboratory. In collaboration with other investigators, the diagnostic techniques available in our laboratory are used to study the epidemiology, economic impact, zoonotic potential, and pathogenesis of the disease in a variety of species (cattle, goats, llamas, etc.). This has led to an increased volume of clinical submissions to the laboratory. In 1995 over 20,000 serum and 5,000 fecal samples were processed for diagnosis and control of Johne's disease. Another focus of the laboratory is investigation of the relationship between Chrohn's disease, a chronic inflammatory bowel disease in humans, and M. paratuberculosis. In addition to diagnostic services, the laboratory provides information and education through telephone consultation, herd visits, veterinary and producer presentations, lay publications and through a Johne's Information Center on the World Wide Web.

Responsibilities

Veterinary Bacteriology

Recent Publications

Sung,N., K. Takayama, and M.T. Collins. 2004. Possible association of GroES and antigen 85 proteins with heat resistance of Mycobacterium paratuberculosis. Appl. Environ. Microbiol. 70:1688-1697.

Carpenter, T.E., I.A. Gardner, M.T. Collins, and R.H. Whitlock. 2004. Effects of prevalence and testing by enzyme-linked immunosorbent assay and fecal culture on the risk of introduction of Mycobacterium avium subsp. paratuberculosis-infected cows into dairy herds. J. Vet. Diagn. Invest. 16:31-38.

Collins, M.T. 2004. Update on paratuberculosis: 3. Control and zoonotic potential. Irish Veterinary Journal 57(1): 49-52.

Collins, M.T. 2003. Update on paratuberculosis: 2. Pathology and diagnosis. Irish Veterinary Journal 56(12): 619-623.

Collins, M.T. 2003. Update on paratuberculosis: 1. Epidemiology of Johne's disease and the biology of Mycobacterium paratuberculosis. Irish Veterinary Journal 56(11):565-574.

Sung, N., and M.T. Collins. 2003. Variation in resistance of Mycobacterium paratuberculosis to acid environments as a function of culture medium. Appl. Env. Microbiol. 69:6833-3840.

Manning, E.J.B., H. Steinberg, V. Krebs, M.T. Collins. 2003. Diagnostic testing patterns of natural Mycobacterium paratuberculosis infection in pygmy goats. Can. J. Vet. Res. 67:213-218.

Manning, E.J.B., T.E. Kucera, N.B. Gates, L.M. Woods, and M. Fallon-McKnight. 2003. Testing for Mycobacterium avium subsp. paratuberculosis infection in asymptomatic free-ranging tule elk from an infected herd. J. Wildlife Diseases 39:323-328.

Collins, M.T. 2003. Summation. pp 531-535 in Proceedings of the 7th International Colloquium on Paratuberculosis. Bilbao, Spain June 12-14, 2002. R.A. Juste, M.V. Geijo, and J.M. Garrido, editors. International Association for Paratuberculosis, Madison, WI USA.

Kramsky, J.A., E.J.B. Manning, and M.T. Collins. 2003. Protein G binding to enriched serum immunoglobulin from nondomestic hoofstock species. J. Vet. Diagn. Invest. 15:253-261.

Manning, E.J.B., M. Augenstein, M.T. Collins, and K.M. Nelson. 2003. Case report - Johne’s disease: the recipient risk. The Bovine Practitioner 37: 20-22.

Quist, C.F., V.F Nettles, E.J.B. Manning, D.G. Hall, J.K. Gaydos, T.J. Wilmers, R.R. Lopez. 2002. Paratuberculosis in key deer (Odocoileus virginianus clavium). J. Wildlife Dis. 38:729-737.

Collins, M.T. 2002. Interpretation of a commercial bovine paratuberculosis enzyme-linked immunosorbent assay by using likelihood ratios. Clin. Diagn. Lab. Immunol. 9:1367-1371.

Zwick, L.S., T.F. Walsh, R. Barbiers, M.T. Collins, M.J. Kinsel, and R.D. Murnane. 2002. Paratuberculosis in a mandrill (Papio sphinx). J. Vet. Diagn. Invest. 14:326-328.

Manning, E. J. B. and M. T. Collins. 2001. Mycobacterium avium subsp paratuberculosis: pathogen, pathogenesis and diagnosis [Review]. Revue Scientifique et Technique 20:133-150.

Kalis, C.H.J., J.W. Hesselink, H.W. Barkema, M.T. Collins. 2001. Use of long-term vaccination with a killed vaccine to prevent fecal shedding of Mycobacterium avium subsp paratuberculosis. Am. J. Vet. Res. 62:270-274.

Kramsky, J.A.*, D.S. Miller, A. Hope, M.T. Collins. 2000. Modification of a bovine ELISA to detect camelid antibodies to Mycobacterium paratuberculosis. Vet. Microbiol. 77:333-337.

Buergelt, C.D., A.W. Layton, P.E. Ginn, M. Taylor, J.M. King, P.L. Haebecker, E. Mauldin, R. Whitlock, C. Rossiter, and M.T. Collins. 2000. The pathology of spontaneous paratuberculosis in North American Bison (Bison bison). Vet. Pathol. 37:428-438.

Miller, D.S., M.T. Collins, B.B. Smith, P.R. Anderson, J. Kramsky, G. Wilder, and A. Hope. Specificity of four serologic assays for Mycobacterium avium subsp. paratuberculosis in llamas and alpacas: a single herd study. J. Vet. Diagn. Invest. 12:345-353, 2000.

Collins, M. T., G. Lisby, C. Moser, D. Chicks, S. Christensen, M. Reichelderfer, N. Høiby, B.A. Harms, Ø.O. Thomsen, U. Skibsted, and V. Binder. Results of multiple diagnostic tests for Mycobacterium avium subsp. paratuberculosis in patients with inflammatory bowel disease and controls. J. Clin. Microbiol. 38:4373-4381, 2000.

Sung, N. and M.T. Collins. Effect of three factors in cheese production (pH, salt, and heat) on Mycobacterium avium subsp. paratuberculosis viability. Appl Env. Microbiol. 66:1334-1339, 2000.

Collins, M.T. 1999. Spreadsheet model for estimating the probability herds are free of paratuberculosis after successive serial tests. pp 66-75 in Proceedings of the 6th International Colloquium on Paratuberculosis. Melbourne, Australia February 14-18, 1999. E.J.B. Manning and M.T. Collins, editors. International Association for Paratuberculosis, Madison, WI USA.

Zhao, B.Y., C.J. Czuprynski, and M.T. Collins. Intracellular fate of Mycobacterium avium subspecies paratuberculosis in monocytes from normal and infected, interferon-responsive cows as determined by a radiometric method. Can.J.Vet.Res. 63:56-61, 1999.

Manning, Elizabeth and Collins, MT. Paratuberculosis in zoo animals. In: Fowler, ME and ER Miller (eds). Zoo and Wild Animal Medicine: Current Therapy 4, W.B. Sauders Company, Philadelphia, 1999. 612-616, 1999.

Sung, N., and M. T. Collins. Thermal tolerance of Mycobacterium paratuberculosis. Appl. Env, Microbiol. 64:999-1005, 1998.

Manning, EJB, Steinberg H, Rossow K, Ruth, GR Collins MT. Epizootic of a paratuberculosis outbreak in farmed elk (Cervus elaphus). J Am Vet Med Assoc. 213, 1320-1322, 1998.

Collins, M.T., and E.J.B. Manning. 1998. Results of serological testing of AABP members for antibody to M. paratuberculosis. Proceedings of the 31st Annual Conference of the American Association of Bovine Practitioners, September 24-26, Spokane Washington.

Cook, W.E., T.E. Cornish, S. Shideler, B. Lasley, and M.T. Collins. Radiometric culture of Mycobacterium avium paratuberculosis from the feces of tule elk. J. Wildlife Dis. 33:635-637, 1997.

Collins, M.T. Clinical approach to control of bovine paratuberculosis. J Am Vet Med Assoc. 204: 208-210, 1994.

Collins, M.T., Sockett, D.C., Goodger, W.J., et al. Herd prevalence, geographic distribution of, and risk factors for, bovine paratuberculosis in Wisconsin. J Am Vet Med Assoc. 204:636-641, 1994.

Collins, M.T. and Sockett, D.C. Accuracy and economics of the USDA-licensed enzyme-linked immunosorbent assay for bovine paratuberculosis. J. Am Vet Med Assoc. 203:1456-1463, 1993.

Collins, M.T., Morgan, I.R. Simulation model of paratuberculosis control in a dairy herd. Prev Vet Med 14:21-32, 1992.