CBS Faculty Office: B29B AHABS

• California-San Francisco, Physiology, Giannini Post-doctoral fellow, 1979-1981
• Colorado State University, Anatomy (with physiology),     Ph.D., 1979
• Colorado State University, Anatomy (with physiology),     Ph.D., 1979
• Colorado State University, Anatomy (with Biochemistry), M.S., 1977
• Colorado State University, Anatomy (with Biochemistry), M.S., 1977
• Colorado State University, Zoology, B.S. ,1972 - 1981 
• EMPLOYMENT HISTORY     

  • Medical, Surgical, Orthopedics Nurse, Loveland Memorial Hospital- McKee Medical Center, Loveland Colorado, 1968-1978                 
  • Histopathology Technician, Loveland Memorial Hosptial-McKee Medical Center, 1974-1978                                           
  • Graduate Research Assistant, Colorado State Univ, Department of Anatomy, Ft Collins, Colorado, 1974-1978 
  • Lecturer in Anatomy, Colorado State Univ, Deparment of Anatomy, Ft Collins, Colorado, 1977-1978                
  • Postdoctoral Research Fellow, University of California-San Francisco, Deparment of Physiology, Mentor: Dr W.F. Ganong, 1978-1981                                                         
  • Visiting Lecturer in Physiology, University of California-San Francisco, Deparment of Physical Therapy, 1980     
  • Assistant Professor, University of Wisconsin,  Department of Comparative Biosciences, School of Veterinary Medicine, 1981-1988  

• CURRENT APPOINTMENTS            

  • Associate Professor, Department of ComparativeBiosciences, School of Veterinary Medicine, Univeristy of Wisconsin-Madison  
  • Affiliate appointments: Department of Comparative Biomedical Sciences, School of Veterinary Medicine and the Neurosciences Training Program, University of Wisconsin, Madison, WI                                                 

The laboratory's primary current interest is to map the involvement of serotoninergic pathways that regulate ingestive physiology of water, electolytes and energy sources.  The broad goal is supported by production of antibodies against serotonin and other relevant neurotransmitters and their receptors for use in light and electron microscopic immunohistochemistry and immunoassays.

Associate Professor

Affiliate Positions: Neuroscience Training Program, Veterinary Science

Nelson DW, Sharp JW, Brownfield MS, Raybould HE, Ney DM.   Localization and activation of glucagon-like peptide-2 receptors on vagal afferents in the rat.  Endocrinology. 2007 May;148(5):1954-62. Epub 2007 Jan 18.  PMID: 17234710 [PubMed - indexed for MEDLINE]

Dimitrov EL, Dejoseph MR, Brownfield MS, Urban JH.  Involvement of neuropeptide Y Y1 receptors in the regulation of neuroendocrine corticotropin releasing hormone neuronal activity.Endocrinology. 2007 Apr 26; [Epub ahead of print] PMID: 17463058 [PubMed - as supplied by publisher]

M. L. Wolak, M. R. DeJoseph, A. D. Cator, A. S. Mokashi, M. S. Brownfield, and J. H. Urban.  Comparative distribution of neuropeptide Y Y1 and Y5 receptors in the rat brain by using immunohistochemistry.  Journal of Comparative Neurology 2003 Sep 22;464 (3):285-311

Parsons, M.J., Benca R.M., Brownfield, M.S., Behan, M.(2001). Age associated changes in the serotonergic system in rat superior colliculus and pretectum. Brain Res Bull. 55:435-444 [Full Text]

Neumaier J.F., Sexton, T.J., Yracheta J., Diaz, A.M., Brownfield, M. 2001. Localization of 5-HT(7) receptors in rat brain by immunocytochemisty, in situ hybridization, and agonist stimulated cFos expression. J of Chemical Neuroanatomy 21(1):63-73

Mitchell, G.S., Bach, K.B., Martin, P.A., Foley, K.T., Olson, E.B., Brownfield, M.S., Miletic, V., Behan, M., McGuirk, S., Sloan, H.E. 2000. Increased spinal monoamine concentrations after chronic thoracic dorsal rhizotomy in goats. J Appl Physiol 89:1266-1274 [Full Text]

Duncan, M.J., Jennes, L., Jefferson, J.B., Brownfield, M.S. (2000). Localization of serotonin (5A) receptors in discrete regions of the circadian timing system in the Syrian hamster. Brain Res 869(1-2):178-185 [Full Text]

Wang, Z.Y., Keith, I.M., Beckman, M.J., Brownfield, M.S., Vidruk, E.H., Bisgard, G.E. 2000. 5-HT5a receptors in the carotid body chemoreception pathway of rat. Neurosci Lett 278:9-12 [Abstract]

Behan, M., Brownfield, M.S. 1999. Age-related changes in serotonin in the hypoglossal nucleus of rat:implications for sleep-disordered breathing. Neurosci Letters 267(2):133-6 [Full Text]

Brownfield, M.S., Yracheta, J., Chu, F., Lorenz, D., Diaz, A. 1998. Functional chemical neuroanatomy of serotonergic neurons and their targets: antibody production and immunohistochemistry (IHC) for 5-HT, it's precursor (5-HTP) and metabolite (5-HIAA), biosynthetic enzyme (TPH), transporter (SERT), and three receptors (5-HT2A, 5-ht5a, 5-HT7). Annals of the N.Y. Acadamy of Sciences 861:232-3 [Full Text]

Van de Kar L.D., Levy, A.D., Li, Q., Brownfield, M.S. 1998. A comparison of the oxytocini and vasopressin responses to the 5-HT1A agonist and potential anxiolytic drug alnespirone (S-20499). Pharmacology, Biochemistry & Behavior 60(3):677-83 [Full Text]

Van de Kar, L.D., Li, Q., Cabrera, T.M., Brownfield, M.S., Battaglia, G. 1998. Alterations in 8-hydroxy-2-(dipropylamino) tetralin-induced neuroendocrine responses after 5,7-dihydroxytryptamine-induced denervation of serotonergic neurons. J of Pharmacology and Experimental Therapeutics. 286(1):256-62. [Full Text]

Turner, D.L., Bach, K.B., Martin, P.A., Olsen, E.B., Brownfield, M., Foley, K.T., Mitchell, G.S. 1997. Modulation of ventilatory control during exercise. Respiration Physiol 110(2-3):277-85

Van De Kar, L.D., Morton, K.D.R. and Brownfield, M.S. Brain catecholamines mediate the delayed reduction in renin release after injection of fenfluramine. Brain Res. 634:77-84, 1994.

Brownfield, M.S., Rittenhouse, P.A., Van De Kar, L.D. and Saydoff, J.A. Brain angiotensin mechanisms mediate serotonin (5HT)-induced secretion of vasopressin, but not oxytocin secretion in conscious male rats. Soc. Neuroscience Abstr . 19:1791, 1993 (Abstract).

Saydoff, J.A., Carnes, M. and Brownfield, M.S. The role of serotoninergic neurons in intravenous hypertonic saline-induced secretion of vasopressin, oxytocin, and ACTH. Brain Res. Bull . 32:567-572, 1993.

Carnes, M., Brownfield, M., Lent, S.J., Nichols, K. and Schuler, L. Pulsatile ACTH and cortisol in goats: Effects of insulin-induced hypoglycemia and dexamethasone. Neuroendocrinology 55:97-104, 1992.

Saydoff, J., Rittenshouse, P., Van De Kar, L. and Brownfield, M. Enhanced serotonergic transmission stimulates oxytocin secretion in conscious male rats. The Journal of Pharmacology and Experimental Therapeutics . 257:95-99, 1991.

Brownfield, M.S., Greathouse, J., Lorens, S.A., Armstrong, J., Urban, J.H. and Van De Kar, L.D. Neuropharmacological characterization of serotoninergic stimulation of vasopressin secretion in conscious rats. Neuroendocrinology. 47:277-283, 1988.