Lab Manual

The following tutorials are designed to teach you the terminology that we use to describe morphologic and biochemical characteristics of bacteria important to veterinary medicine. During the final laboratory practical exam you may be asked to identify structures, physiological properties, as well as identify organisms and cultures based on veterinary case studies.

Introduction to identification of bacterial organisms

		The Gram stain
		Colony morphology and hemolysis
		Biochemical tests

Chart 1: Staphylococci

		The cellular arrangement of Staphylococcus sp.
		Colony morphology of Staphylococcus sp.
		Typical hemolysis characteristics of Staphylococcus sp.
		Differentiation of Staphylococcus sp. and Streptococcus sp. using the catalase test
		Differentiation of Staphylococcus sp. using the coagulase test

Chart 2: Streptococci

		The cellular arrangement of Streptococcus sp.
		Colony morphology of Streptococcus sp.
		Typical hemolysis characteristics of Streptococcus sp.
		Differentiation of Staphylococcus sp. and Streptococcus sp. using the catalase test
		The CAMP test
		The esculin test
		The CTA sugar test
		Antibiotic sensitivity tests

Chart 3: The C-L-E-A-R Organisms

		The cellular arrangement of the C-L-E-A-R bacteria
		Colony morphology of C-L-E-A-R bacteria
		Differentiation of C-L-E-A-R organisms using the room temperature motility test
		Differentiation of C-L-E-A-R organisms using the hydrogen sulfide production test
		Differentiation of C-L-E-A-R organisms using the catalase production test

Chart 4: The Enteric Rods

		The cellular arrangement of enteric rods
		Colony morphology of enteric rods
		The MacConkey agar test
		Selenite media and the XLD agar test
		The oxidase test
		Differentiation of enteric rods using triple sugar iron agar (TSI) test
		Differentiation of enteric rods using the indole test
		Differentiation of enteric rods using the citrate test
		Differentiation of enteric rods using the urease test
		Differentiation of enteric rods using the ornithne decarboxylase test

Chart 5: The Non-Enteric Rods

		Cellular Morphology
		Colony morphology of non-enteric Gram-negative rods - 1
		Colony morphology of non-enteric Gram-negative rods - 2
		The MacConkey agar test
		The oxidase test
		Differentiation of non-enteric rods using triple sugar iron agar (TSI) test
		Differentiation of non-enteric rods using the indole test
		Differentiation of non-enteric rods using the citrate test
		Differentiation of non-enteric rods using the urease test
		Differentiation of non-enteric rods using the ornithne decarboxylase test

Large Gram-Positive Rods

		Cellular morphology
		Colony morphology of Bacillus

Strict Anaerobes

		The Gas-Pak Jar
		Thioglycollate Broth
		Cellular and Colony morphology of Clostridium perfringens
		Cellular and Colony morphology of Actinomyces bovis
		Cellular and Colony morphology of Bacteroides
		Cellular and Colony morphology of Fusobacterium