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Streptococcus: CAMP test

Gram stain
The CAMP test is used to identify Streptococcus agalactiae, one of the most important causes of mastitis in dairy cattle. When performed on streptococci isolated from the udder of cattle, only Streptococcus agalactiae will give the characteristic arrow head hemolysis at the junction of the Staphylococcus aureus and Streptococcus agalactiae streaks.

On this slide, the Staphylococcus aureus streak runs vertically, and the horizontal streak on the left is CAMP positive - Streptoccus agalactiae. Note the arrowhead shaped area of hemolysis. The horozontal streak on the right is a CAMP negative streptococcal species.