Dr. K. D. Kroening

K. Dubear Kroening, Ph.D.

DrDubear@aol.com
dkruening@uwc.edu

Education

Ph.D. Biochemistry, Molecular Biology, and Biophysics, 1989-1995
     University of Minnesota, St. Paul, MN Department of Biochemistry

M.S. Biology - work, 1986-1989
     Northern Michigan University, Marquette, MI Department of Biology

B.S. Water Science, 1982-1986
     Northern Michigan University, Marquette, MI Departments of Biology and Chemistry

Academic Employment

Assistant Professor - University of Wisconsin - Fox Valley 2000 - present

Post-doctoral Research – School of Veterinary Medicine June ’98 - 2000

- School of Pharmacy, University of Wisconsin April ’97 - 2000

- Department of Chemistry, University of Wisconsin Jan. ’95 - March ’97

Research Assistant - Department of Biochemistry, University of Minnesota July ’89 - Dec. ’94

Instructor - Introductory Chemistry - College of St. Catherine Spring, Fall ’93

Teaching Assistant

University of Minnesota
Fall, ’90 and ’91

DNA Recombinant Laboratory

Northern Michigan University

Medical Microbiology Laboratory
Human Physiology Laboratory
Biometrics
Human Anatomy and Physiology Laboratory
Fall ’88, Summer ’89
Winter ’89
Winter ’89
Fall ’87, Winter ’88

Current and Previous Research

Prior to leaving UW-Madison, I was working with Dr. John Oaks in an attempt to identify and isolate the signal factor(s) that are responsible for the tapeworm’s ability to alter intestinal motility. This work includes utilizing tapeworm fractions for bioassaying rats implanted with intestinal electrodes and a cannula.

Prior to coming to Dr. Oaks’ laboratory, I worked with Dr. William Devane in the School of Pharmacy attempting to identify cannabinoid-responsive genes in mice utilizing differential display and reverse transcriptase - polymerase chain reaction (DDRT-PCR). Subsequent cloning, sequencing and Northern blotting allowed for analysis and databank searching of candidate products.

Prior to my work with Dr. Devane I worked with Dr. Richard Guilfoyle in Dr. Lloyd Smith’s laboratory on technology development to aid in the streamlining of the front-end portion of the Human Genome Project. This included the construction of a fesmid vector with solid phase capture capabilities along with the development and optimization of a strategy which provides for the amplification of specific fragments from a class-II restriction endonuclease digestion utilizing extra long PCR.

My Ph.D. work in the laboratory of Dr. David Bernlohr consisted of developing a new project that generated yeast mutants defective in the ability to regulate certain genes in the presence of unsaturated fatty acids. The subsequent rescue of the mutation and cloning of yeast genomic DNA which corrected the defect. The identification and analysis of the resultant subclone in addition to extensive analysis of the upstream region of one of the subclones in order to examine its regulation.

Techniques / Instrumentation

Molecular Biology: Differential Display, Hybridizations (Northerns, Southerns, Glass Supports-DNA Chips), Cloning, Sequencing (using radioisotopes and dye labeled nucleotides and primers), Mutagenesis, Purifications (including magnetic bead technology), Vector Construction, PCR (normal and XL-PCR), RecA Manipulations, Cell Culturing.

Protein: Gel Electrophoresis, Purification, Biochemical Assays, Westerns

Instrumentation: GC/MS, Fluoroimager, Spectrophotometers, HPLC, IR, NMR, Computers (including databank searching and molecular programs) ABI373, GenomyxLR DNA Sequencer.

Publications:

Kroening, K. D., N. Zimmerman, P. Bass and J. A. OAKS. 2003. Guanosine 3'-5'-cyclic monophosphate: A tapeworm-secreted signal molecule communicating with the host's intestine. J. Parasitology 89:1136-1141

Kroening, K.D and Devane, W.A. Identification, Cloning and Sequencing of a Novel Cannabinoid-Induced Gene Using Differential Display. (in preparation)

Liu, Q., Kroening, K.D., and Guilfoyle, R.A. 1998. Enhanced Specificity of Ligation-mediated PCR Amplification of Specific Class-II Restriction Endonuclease Fragments Using the Universal Base Analogue 3-Nitropyrrole. (submitted)

Guilfoyle, R.A., Leeck, C.L., Kroening, K.D., Smith, L.M., and Guo, Z. 1997. Ligation-Mediated PCR Amplification of Specific Fragments from a Class-II Restriction Endonuclease Total Digest. Nucleic Acids Res. 25(9): 1854-1858.

McHale, M.W., Kroening, K.D., and Bernlohr, D.A. 1996. Identification of a Class of Saccharomyces cerevisiae Mutants Defective in Fatty Acid Repression of Gene Transcription and Analysis of the FRM2 Gene. Yeast 12: 319-331.

Abstracts:

Guo, Z., Kroening, K.D., Hayden, J.M., Smith, L.M., and Guilfoyle, R.A. 1995. Feasibility of Performing "Restriction Hybridization Ordering" (RHO) on Glass Supports. Genome Sci. and Technol. 1(1): P-55.

Hayden, J., Guo, Z., Kroening, K.D., Smith, L.M., and Guilfoyle, R.A. 1995. "Restriction Hybridization Ordering" (RHO): A Front-end Assembly Strategy for Low-Pass, High-Coverage Sequencing of Shotgun Cloned DNA. Genome Sci. and Technol. 1(1): P-56.

Kroening, K.D., McHale, M.W., Magee, P.T., and Bernlohr, D.A. 1993. Fatty Acid Control of Gene Expression: Identification of Saccharomyces cerevisiae Mutants Defective in Repression of Stearoyl-Coenzyme A Desaturase by Arachidonic Acid. The FASEB Journal 7(7): A1224.

McHale, M.W., Kroening, K.D., Magee, P.T., and Bernlohr, D.A. 1993. Isolation and Characterization of Fatty Acid Response Mutants in Saccharomyces cerevisiae. Genetics Society of America - Yeast Genetics Meeting, Madison, WI.

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